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detergent compatible rcdc protein assay kit  (Bio-Rad)


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    Structured Review

    Bio-Rad detergent compatible rcdc protein assay kit
    Detergent Compatible Rcdc Protein Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 16487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/detergent+compatible+rcdc+protein+assay+kit/DC+Protein+Assay+Kit+I/pmc11287154__pnas__2313609121__sapp-92-9-14
    Average 97 stars, based on 16487 article reviews
    detergent compatible rcdc protein assay kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Homogenization:

    Article Title: Liver proteomics in progressive alcoholic steatosis
    Article Snippet: Frozen liver tissue samples (75 mg, in triplicate for each animal and three animals from each control and ethanol-fed group at each time point) were homogenized in 500 μl DeStreak rehydration buffer (GE Healthcare Lifesciences, Pittsburg, PA) with protease inhibitor cocktail (Sigma-Aldrich, Milwaukee, WI). .. Following homogenization, benzonase (Sigma-Aldrich, Milwaukee, WI) was added to the homogenates and the mixture incubated at room temperature for 1 h. The homogenates were centrifuged at 13,000 g for 15 minutes at 25° C. The resulting supernatant was collected and the protein concentrations were estimated using the reducing agent and detergent compatible (RCDC) protein assay kit (BioRad, Hercules, CA) and the samples were stored at −80° C until further analysis. ..

    Incubation:

    Article Title: Liver proteomics in progressive alcoholic steatosis
    Article Snippet: Frozen liver tissue samples (75 mg, in triplicate for each animal and three animals from each control and ethanol-fed group at each time point) were homogenized in 500 μl DeStreak rehydration buffer (GE Healthcare Lifesciences, Pittsburg, PA) with protease inhibitor cocktail (Sigma-Aldrich, Milwaukee, WI). .. Following homogenization, benzonase (Sigma-Aldrich, Milwaukee, WI) was added to the homogenates and the mixture incubated at room temperature for 1 h. The homogenates were centrifuged at 13,000 g for 15 minutes at 25° C. The resulting supernatant was collected and the protein concentrations were estimated using the reducing agent and detergent compatible (RCDC) protein assay kit (BioRad, Hercules, CA) and the samples were stored at −80° C until further analysis. ..

    Protein Concentration:

    Article Title: Mfn2-dependent fusion pathway of PE-enriched micron-sized vesicles
    Article Snippet: .. Inset: protein concentration of the collected fractions determined by detergent compatible RCDC-Protein assay kit (BioRAD). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: The unfolded protein response of the endoplasmic reticulum protects Caenorhabditis elegans against DNA damage caused by stalled replication forks
    Article Snippet: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251). .. Protein concentrations were determined using the reducing agent and detergent compatible (RCDC) protein assay kit (Bio-Rad #500-0121), and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoblotting were performed as described ( Hou et al . 2014 ), using anti-Ser51-Phospho-eIF2α rabbit antibody (Cell Signaling Technologies #9721), anti-α-tubulin mouse antibody (Sigma #T9026), and anti-rabbit HRP-conjugated (New England Biolabs [NEB] #7074) and anti-mouse HRP-conjugated (Cell Signaling Technologies #7076) secondary antibodies. .. Detection was done using ECL (Pierce #32109).

    Western Blot:

    Article Title: The unfolded protein response of the endoplasmic reticulum protects Caenorhabditis elegans against DNA damage caused by stalled replication forks
    Article Snippet: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251). .. Protein concentrations were determined using the reducing agent and detergent compatible (RCDC) protein assay kit (Bio-Rad #500-0121), and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoblotting were performed as described ( Hou et al . 2014 ), using anti-Ser51-Phospho-eIF2α rabbit antibody (Cell Signaling Technologies #9721), anti-α-tubulin mouse antibody (Sigma #T9026), and anti-rabbit HRP-conjugated (New England Biolabs [NEB] #7074) and anti-mouse HRP-conjugated (Cell Signaling Technologies #7076) secondary antibodies. .. Detection was done using ECL (Pierce #32109).



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